CD45 Antibody (Leukocyte marker)

• Catalog Number: orb606681
• Category: Antibodies
• Description: CD45, also referred to as CD45R and PTPRC (Protein tyrosine phosphatase receptor type C), has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Along with other members of the PTP family, it regulates a number of cellular processes including cell differentiation, growth and mitotic cycle, and is an essential regulator of B- and T-cell antigen receptor-mediated activation. Multiple isoforms of CD45 are distributed throughout the immune system and arise due to alternative splicing of exons located in the N-terminus. CD45RA contains the A exon and is a naive T-cell marker which may help prevent autoimmune disease. CD45RB contains B and stains most leukemias and lymphomas. CD45RC contains C and stains thymocytes, monocytes and dendritic cells. CD45RO doesn't contain A, B or C and is a marker of activated T-cells that can be used to classify and diagnose and classify lymphomas. Clone 2B11 antibody will bind to all CD45 isoforms. The variation in these isoforms is localized to the extracellular domain, with the intracellular domain being conserved. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: Bra55
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: REH cells (a non-T, non-B acute lymphoblastic leukaemia cell line) were used as the immunogen for the CD45 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CD45 antibody is available for research use only.
• UniProt ID: P08575
• Storage: CD45 antibody (with azide) can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Due to variation in protocol and secondary antibody used, the CD45 antibody may need to be titered for optimal performance.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human tonsil tissue with CD45 antibody. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with CD45 antibody. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Immunofluorescent staining of PFA-fixed human Jurkat cells with CD45 antibody (clone BRA55, green) and Reddot nuclear stain (red).

Flow cytometry testing of PFA-fixed human Jurkat cells with CD45 antibody (clone BRA55); Red = isotype control, Blue = CD45 antibody.

SDS-PAGE analysis of purified, BSA-free CD45 antibody (clone Bra55) as confirmation of integrity and purity.