beta 2 Microglobulin/B2m Antibody

• Catalog Number: orb614096
• Category: Antibodies
• Description: beta 2 Microglobulin/B2m Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, WB
• Reactivity: Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived rat beta 2 Microglobulin/B2m recombinant protein (Position: I21-M119).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.25μg/ml, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5μg/ml, Mouse, Rat Flow Cytometry, 1-3μg/1x106 cells, Rat Direct ELISA, 0.1-0.5μg/ml, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 13 kDa
• UniProt ID: P01887
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Beta-2-microglobulin; B2m
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of RH35 cells using anti-Beta 2 Microglobulin/B2m antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG (1μg/1x10^6).

IF analysis of Beta 2 Microglobulin/B2m using anti-Beta 2 Microglobulin/B2m antibody.

IF analysis of Beta 2 Microglobulin/B2m using anti-Beta 2 Microglobulin/B2m antibody.

WB analysis using anti-Beta 2 Microglobulin/B2m antibody.Lane 1:rat thymus tissue, Lane 2:rat lung tissue, Lane 3:rat spleen tissue, Lane 4:rat stomach tissue, Lane 5:rat small intestine tissue, Lane 6:mouse thymus tissue.