Beta 2 Microglobulin/B2M Antibody

• Catalog Number: orb18580
• Category: Antibodies
• Description: Beta 2 Microglobulin/B2M Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Beta 2 Microglobulin recombinant protein (Position: Q22-M119). Human Beta 2 Microglobulin shares 69.4% and 74.5% amino acid (aa) sequence identity with mouse and rat Beta 2 Microglobulin, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry(Paraffin-embedded Section), 0.5-1 μg/ml, Human, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human ELISA(Cap), 1-5 μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 13 kDa
• UniProt ID: P61769
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Adding 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U251 cells using anti- Beta 2 Microglobulin antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Beta 2 Microglobulin using anti-Beta 2 Microglobulin antibody.Lane 1:HEPG2 cell; 2:22RV1 cell.

IF analysis of Beta 2 Microglobulin using anti- Beta 2 Microglobulin antibody. Beta 2 Microglobulin was detected in immunocytochemical section of A431 cell.

IF analysis of Beta 2 Microglobulin using anti- Beta 2 Microglobulin antibody. Beta 2 Microglobulin was detected in immunocytochemical section of A431 cell.

Sandwich ELISA - Recombinant human Beta 2 Microglobulin/B2M protein standard curve.Use in combination with reagents from Human Beta 2 Microglobulin/B2M ELISA Kit EZ-Set (DIY Antibody Pairs).

IHC analysis of Beta 2 Microglobulin using anti- Beta 2 Microglobulin antibody. Beta 2 Microglobulin was detected in paraffin-embedded section of human intestinal cancer tissues.

IHC analysis of Beta 2 Microglobulin using anti- Beta 2 Microglobulin antibody. Beta 2 Microglobulin was detected in paraffin-embedded section of human lung cancer tissues.