Beta 2 Microglobulin B2M Antibody (monoclonal, 2H10)

• Catalog Number: orb507558
• Category: Antibodies
• Description: Beta 2 Microglobulin B2M Antibody (monoclonal, 2H10)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2H10
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Monkey
• Isotype: Mouse IgG2b
• Immunogen: E.coli-derived human Beta 2 Microglobulin recombinant protein (Position: Q22-M119). Human Beta 2 Microglobulin shares 69.4% and 74.5% amino acid (aa) sequence identity with mouse and rat Beta 2 Microglobulin, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 12 kDa
• UniProt ID: P61769
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Beta-2-microglobulin; Beta-2-microglobulin form pI
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A431 cells using anti-Beta 2 Microglobulin antibody (Blue line).Isotype control antibody (Green line) was mouse IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Beta 2 Microglobulin using anti-Beta 2 Microglobulin antibody.Lane 1:COS-7 cell; 2:HELA cell; 3:HL-60 cell; 4:HEPG2 cell; 5:K562 cell; 6:293T cell.

IF analysis of Beta 2 Microglobulin using anti-Beta 2 Microglobulin antibody. Beta 2 Microglobulin was detected in immunocytochemical section of A431 cells.

IHC analysis of Beta 2 Microglobulin using anti-Beta 2 Microglobulin antibody. Beta 2 Microglobulin was detected in paraffin-embedded section of human mammary cancer tissue.

IHC analysis of Beta 2 Microglobulin using anti-Beta 2 Microglobulin antibody. Beta 2 Microglobulin was detected in paraffin-embedded section of human intestinal cancer tissue.

IHC analysis of Beta 2 Microglobulin using anti-Beta 2 Microglobulin antibody. Beta 2 Microglobulin was detected in paraffin-embedded section of human lung cancer tissue.