Beta-2-Microglobulin antibody

• Catalog Number: orb344600
• Category: Antibodies
• Description: Beta-2-Microglobulin antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: beta-2-Microglobulin [Human Urine]
• Concentration: 1.0 mg/ml
• Dilution range: ELISA: 1:2,000 - 1:10,000, IHC: 1:500 - 1:2,000, IF: 1:200, WB: 1:1,000 - 1:5,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-beta-2-Microglobulin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified b2-Microglobulin [Human Urine]. Cross reactivity against b2-Microglobulin from other sources is unknown.
• Conjugation: Unconjugated
• UniProt ID: P61769
• NCBI: NP_004039.1
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Beta-2-Microglobulin Antibody, b2-Micr
• Note: For research use only
• Application notes: Anti-beta-2-Microglobulin antibody has been tested in western blot, immunohistochemistry, and immunofluorescence. This product detects a single band of the expected apparent molecular weight (~13.7kDa), and is suitable for use in ELISA. Researchers should determine optimal titers for applications that are not stated below.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of Human Embryonic Kidney cells (HEK 293 cells) using Beta-2-Microglobulin antibody

Immunohistochemical staining of normal human skin tissue using Beta-2-Microglobulin antibody