B2M Antibody

SKU: orb344599

Description

Beta-2-Microglobulin antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution Range: ELISA: 1:2,000 - 1:10,000, IHC: 1:500 - 1:2,000, IF: 1:200, WB: 1:200 - 1:500
• Reactivity: Human
• Application Notes:
  Anti-beta-2-Microglobulin antibody has been tested in western blot, immunohistochemistry, and immunofluorescence. This product detects a single band of the expected apparent molecular weight (~13.7kDa), and is suitable for use in ELISA. Researchers should determine optimal titers for applications that are not stated below.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-Beta-2-Microglobulin Antibody was produced by repeated immunizations with human beta-2-Microglobulin protein isolated from urine.
• Target: B2M
• Purity: Anti-beta-2-Microglobulin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Rabbit Serum, as well as purified and partially purified b2-Microglobulin (Human Urine). Cross reactivity against b2-Microglobulin from other sources is unknown.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Beta-2-Microglobulin Antibody, b2-Microglobulin, B2M antibody, B2MG_HUMAN antibody, Beta 2 microglobin antibody, Beta 2 microglobulin precursor antibody, Beta chain of mhc class 1 proteins antibody, Hdcma22p antibody, CDABP0092 antibody, IMD43 antibody

Quick Database Links

Gene Symbol

B2M

UniProt

P61769

RefSeq (Protein):NP_004039.1