ACAP1 (phospho Ser554) rabbit pAb

• Catalog Number: orb770970
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ACAP1 (phospho-Ser554)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human Centaurin-beta1 around the phosphorylation site of Ser554. AA range:520-569
• MW: 82kD
• Tested applications: ELISA, IHC-P
• Dilution range: Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-ACAP1 antibody, anti-CENTB1 antibody, anti-KI
• Note: For research use only

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Western Blot analysis of Hela treated or untreated by LPS lysis, using primary antibody at 1:1000 dilution. Secondary antibody was diluted at 1:10000.