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    UBE2L3 antibody

    Catalog Number: orb382106

    DispatchUsually dispatched within 5-10 working days
    $ 413.00
    Catalog Numberorb382106
    CategoryAntibodies
    DescriptionRabbit polyclonal antibody to UBE2L3.
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsIF, IH, WB
    ReactivityHuman, Mouse, Rat
    ImmunogenRecombinant full length protein of human UBCH7
    Dilution rangeWB: 1:1000-2000, IHC-P: 1:50-200
    Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    ConjugationUnconjugated
    TargetUBE2L3
    Entrez22195, 7332, 100042355
    UniProt IDP68037, P68036
    SourceRabbit
    StorageShipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
    Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    Alternative namesUBCE7; UBCH7; Ubiquitin-conjugating enzyme E2 L3;
    Read more...
    NoteFor research use only
    Expiration Date12 months from date of receipt.
    UBE2L3 antibody

    Western blot analysis of UBCH7 expression in A549 (A), K562 (B), mouse testis (C), mouse brain (D) whole cell lysates. (Predicted band size: 14; 17; 24 kD; Observed band size: 18 kD)

    UBE2L3 antibody

    Immunohistochemical analysis of UBCH7 staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    UBE2L3 antibody

    Immunofluorescent analysis of UBCH7 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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