UBE2L3 Antibody

• Catalog Number: orb1257744
• Category: Antibodies
• Description: UBE2L3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 73-212 of human UBE2L3 (NP_001243284.1).
• Concentration: batch dependent
• Dilution range: WB: 1:1000 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 18kDa
• Target: UBE2L3
• UniProt ID: P68036
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: UBE2L3, E2-F1, L-UBC, UBCH7, UbcM4
• Note: For research use only
• Application notes: WB: 1:1000 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using UBE2L3 antibody (orb1257744) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Immunohistochemistry of paraffin-embedded rat heart using UBE2L3 antibody (orb1257744) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using UBE2L3 antibody (orb1257744) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using UBE2L3 antibody (orb1257744) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using UBE2L3 Polyclonal Antibody (orb1257744) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using UBE2L3 Polyclonal Antibody (orb1257744) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.