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UBE2L3 antibody

Catalog Number: orb18828

DispatchUsually dispatched within 5-10 working days
$ 500.00
Catalog Numberorb18828
CategoryAntibodies
DescriptionGoat polyclonal antibody to UBE2L3
Species/HostGoat
ClonalityPolyclonal
Tested applicationsELISA, IF, WB
ReactivityBovine, Human, Mouse, Porcine, Rat
Dilution rangeELISA: 1:128000, WB: 1-3 μg/ml, IHC-P: 5 μg/ml
ConjugationUnconjugated
MW17.9
TargetUBE2L3
Entrez7332
Protein SequenceEFTKKYGEKRPVD
RRIDAB_10749778
StorageAliquot and store at -20°C. Minimize freezing and thawing.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Alternative namesanti UBE2L3 antibody, anti ubiquitin-conjugating e
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NoteFor research use only
Application notesELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 18kDa band observed in Hela and Jurkat lysates (predicted MW of 18kDa according to NP_003338). Recommended for use at 0.5-2 μg/ml.
Expiration Date12 months from date of receipt.
UBE2L3 antibody

EB05770 (0.01µg/ml) staining of HEK293 (A), HepG2 (B) and Jurkat (C) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

UBE2L3 antibody

EB05770 (0.03µg/ml) staining of Human (A), Mouse (B) and Rat (C) Skeletal Muscle lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

UBE2L3 antibody

Immunofluorescence staining of MCF7 (A) and Hela (B) cells with 5ug/ml EB05770 antibody. Detected with Rabbit anti-goat IgG-Alexafluor488 antibody at 1:1000. Nuclei Counterstained with DAPI.

UBE2L3 antibody

EB05770 (0.01µg/ml) staining of Human (A), Mouse (B), Rat (C) and Pig (D) Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

UBE2L3 antibody

EB05770 staining (0.5µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

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