UBE2L3 Antibody

• Catalog Number: orb1247875
• Category: Antibodies
• Description: UBE2L3 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse, Porcine, Rat
• Immunogen: The immunogen for this antibody is: C-EFTKKYGEKRPVD
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 18kDa band observed in lysates of cell lines HeLa HEK293, HepG2 and Jurkat and also in lysates of Human, Mouse and Rat Heart, Skeletal Muscle and Testis and of Pig Heart (calculated MW of 17.9kDa according to Human NP_003338.1 and Mouse NP_033482.1). Recommended concentration: 1-3ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm. Dividing cells appear to show enhanced expression. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: UBE2L3
• UniProt ID: P68036
• NCBI: NP_003338.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: UBE2L3, ubiquitin-conjugating enzyme E2L 3, UBCH7,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 18kDa band observed in lysates of cell lines HeLa HEK293, HepG2 and Jurkat and also in lysates of Human, Mouse and Rat Heart, Skeletal Muscle and Testis and of Pig Heart (calculated MW of 17.9kDa according to Human NP_003338.1 and Mouse NP_033482.1). Recommended concentration: 1-3ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm. Dividing cells appear to show enhanced expression. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247875 staining (0.5 ug/ml) of HeLa lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb1247875 (0.01 ug/ml) staining of Human (A), Mouse (B), Rat (C) and Pig (D) Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247875 (0.03 ug/ml) staining of Human (A), Mouse (B) and Rat (C) Skeletal Muscle lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247875 (0.01 ug/ml) staining of HEK293 (A), HepG2 (B) and Jurkat (C) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunofluorescence staining of MCF7 (A) and HeLa (B) cells with 5 ug/ml orb1247875 antibody. Detected with Rabbit anti-goat IgG-Alexafluor488 antibody at 1:1000. Nuclei Counterstained with DAPI.