ubiquitin conjugating enzyme E2 L3 Antibody

• Catalog Number: orb556196
• Category: Antibodies
• Description: Rabbit polyclonal antibody to UBE2L3
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC-P, IP, WB
• Reactivity: Human, Mouse, Zebrafish
• Isotype: IgG
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human UBE2L3. The exact sequence is proprietary.
• Concentration: 1 mg/ml
• Dilution range: WB: 1:500-1:3000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000 , IP: 1:100-1:500
• Form/Appearance: Liquid: 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Purity: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• MW: 18
• Target: UBE2L3
• UniProt ID: P68036
• Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles.
• Buffer/Preservatives: 0.01% Thimerosal. 1XPBS (pH7), 40% Glycerol
• Alternative names: ubiquitin conjugating enzyme E2 L3 , E2-F1 , L-UBC
• Note: For research use only
• Application notes: Positive control: Neuro2A , GL261 , C8D30 , HeLa , HepG2 , mouse testis
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of HeLa cells using UBE2L3 antibody

Immunohistochemical staining of A549 xenograft using UBE2L3 antibody

Immunoprecipitation analysis of HeLa Whole cell using UBE2L3 antibody

Western blot analysis of Hela(Lane 1), Hep G2(Lane 2) using UBE2L3 antibody

Western blot analysis of Hela(Lane 1), Hep G2(Lane 2) using UBE2L3 antibody

Western blot analysis of Hela(Lane 1), Hep G2(Lane 2) using UBE2L3 antibody

Western blot analysis of Hela(Lane 1), Hep G2(Lane 2) using UBE2L3 antibody