UBC antibody

• Catalog Number: orb154291
• Category: Antibodies
• Description: Rabbit polyclonal antibody to UBC. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target proteinubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the ubiquitinactivating enzyme (UBE1 or E1). The activated ubiquitin is subsequently transferred to the ubiquitincarrier protein E2, and then from E2 to ubiquitin ligase E3 for final delivery to the amino group of the target protein lysine residue (13). Combinatorial interactions of different E2 and E3 proteins result in substrate specificity (4). UBE1 has two isofoms: UBE1a is a nuclear protein of 117 kDa while UBE1b is a nuclear and cytoplasmic protein of 110 kDa
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide of human UBC(NP_066289.3).
• Dilution range: WB: 1:1000-2000, IHC-P: 1:50-100
• Conjugation: Unconjugated
• MW: 12-55kDa
• UniProt ID: P0CG48
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Alternative names: anti UBC antibody, anti HMG20 antibody, anti Ubiqu
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of HepG2 and HeLa cell line using UBC antibody

Immunohistochemical staining of human liver tissue using UBC antibody (dilution of 1:100)

Immunohistochemistry analysis of human breast carcinoma tissue using UBC antibody