TNF antibody

• Catalog Number: orb750677
• Category: Antibodies
• Description: TNF antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, WB
• Reactivity: Human, Primate
• Isotype: Antiserum
• Immunogen: The whole rabbit serum was prepared by repeated immunizations with recombinant human TNFa produced in E.coli.
• Concentration: 75 mg/mL
• Dilution range: ELISA: 1:1,000 - 1:5,000, IHC: 1:100 - 1:500, IF: 1:100, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This antiserum has been heated to 56°C for 30 minutes. The antiserum is directed against mature 17,000 MW human TNFa and is useful in determining its presence in various assays. In general, this antibody also detects primate TNFa in the same formats using similar dilutions. The antibody does not recognize human TNFb (lymphotoxin). This antiserum will recognize the cell-bound precursor of TNFa as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFa activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
• Conjugation: Unconjugated
• UniProt ID: P01375
• NCBI: P01375.1
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: None. None
• Alternative names: APC1 antibody, Cachectin antibody, DIF antibody, D
• Note: For research use only
• Application notes: Anti-Human TNFα has been tested for use in immunohistochemistry, immunofluorescence, and immunoblotting. It recognizes the 17,000 MW TNFα. Reactivity in other immunoassays is unknown.
• Expiration Date: 12 months from date of receipt.