TNF alpha antibody

• Catalog Number: orb348870
• Category: Antibodies
• Description: Human monoclonal antibody to TNF alpha
• Species/Host: Human
• Clonality: Monoclonal
• Clone Number: CDP 571 (Humicade)
• Tested applications: ELISA
• Reactivity: Human
• Isotype: IgG4
• Immunogen: Human TNF-alpha
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: TNF alpha
• UniProt ID: P01375
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: TNFA; Tumour necrosis factor superfamily member 2;
• Note: For research use only
• Application notes: The antibody binds specifically to TNF-alpha, a pro-inflammatory cytokine which stimulates release of other cytiokines e.g. IL-1, IL-6 and GM-CSF as well as secretion of ICAM-1 and VCAM-1 from endothelial cells. It stimulates acute phase responses and acts as a chemoattractant for NK cells and monocytes/macrophages. The antibody reached Phase II clinical trials with Celltech, UCB.
• Expiration Date: 12 months from date of receipt.

References

Stephens, S. et al. Comprehensive pharmacokinetics of a humanized antibody and analysis of residual anti-idiotypic responses Immunology, 85, 668-674 (1995)

PubMed: 7558164

Immunohistochemical staining of human thyroid tumour tissue using TNF alpha antibody (dilution of primary antibody - 5 ug/ml)

Immunohistochemical staining of paraffin embedded mouse testis tissue using anti-TNF alpha (primary antibody at 5 ug/ml)

IHC-P staining of rat spinal cord tissue using TNF alpha antibody (dilution at 5 ug/ml)

Immunohistochemical staining of paraffin embedded rat liver tissue using TNF alpha antibody (primary antibody at 5 ug/ml)

IHC-P image of rat large intestines tissue using anti-TNF alpha (dilution of primary antibody at 5 ug/ml)

Western blot analysis of rat liver (lane 1), rat spinal cord (lane 2), mouse testis (lane 3), rat colon (lane 4); human thyroid tumour (lane 5) using TNF alpha antibody (1 ug/ml)