DR5/TNFRSF10B Antibody

• Catalog Number: orb389514
• Category: Antibodies
• Description: DR5/TNFRSF10B Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human DR5 recombinant protein (Position: K233-S440). Human DR5 shares 48.4% amino acid (aa) sequence identity with mouse DR5.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 47878 MW
• UniProt ID: O14763
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Tumor necrosis factor receptor superfamily member
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A549 cells using anti-DR5 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of U87 cells using anti-DR5 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of THP-1 cells using anti-DR5 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of PC-3 cells using anti-DR5 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of DR5 using anti-DR5 antibody.Lane 1:rat thymus tissue;2:mouse cardiac muscle tissue;3:human K562 cell.

IHC analysis of DR5 using anti-DR5 antibody. DR5 was detected in a paraffin-embedded section of rat kidney tissue.

IHC analysis of DR5 using anti-DR5 antibody. DR5 was detected in a paraffin-embedded section of mouse intestine tissue.

IHC analysis of DR5 using anti-DR5 antibody. DR5 was detected in a paraffin-embedded section of human intetsinal cancer tissue.