TNF Antibody

• Catalog Number: orb1272262
• Category: Antibodies
• Description: TNF Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2.1_4E10-1H11
• Tested applications: ELISA, NeA, WB
• Reactivity: Human
• Immunogen: The monoclonal antibody was produced in BALB/c X ICR F1 mice using recombinant hTNF-alpha as the immunizing antigen.
• Concentration: batch dependent
• Dilution range: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hTNF-α (0.5 ng/mL), a concentration of ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human TNF-α when used with our biotinylated antigen affinity purified anti-human TNF-α (XP-5282Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.Western blot:To detect hTNF-α by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTNF-α is 0.5-1.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: TNF
• UniProt ID: P01375
• NCBI: P01375
• Storage: TNF-α lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.
• Alternative names: tumor necrosis factor alpha, TNFA, TNFSF2, Tumor n
• Note: For research use only
• Application notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hTNF-α (0.5 ng/mL), a concentration of < 0.1 μg/mL of this antibody is required.ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human TNF-α when used with our biotinylated antigen affinity purified anti-human TNF-α (XP-5282Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.Western blot:To detect hTNF-α by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTNF-α is 0.5-1.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 15.0-20.0 ug/ml overnight at 4°C. An HRP-labeled polymer detection system was used with DAB Chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.

This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 15.0-20.0 ug/ml overnight at 4°C. An HRP-labeled polymer detection system was used with DAB Chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.