TNF Antibody

• Catalog Number: orb1252005
• Category: Antibodies
• Description: TNF Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: TMDa1-1
• Tested applications: IF, IHC-P
• Reactivity: Human, Mouse, Rat
• Isotype: IgM, kappa
• Immunogen: Full length human recombinant protein was used as the immunogen for the TNF alpha antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Immunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 2-4 ug/ml for 30 min at RT (1)Titering of the TNF alpha antibody may be required for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: TNF
• UniProt ID: P01375
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Tumor necrosis factor, Cachectin, TNF-alpha, Tumor
• Note: For research use only
• Application notes: Immunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 2-4 ug/ml for 30 min at RT (1)Titering of the TNF alpha antibody may be required for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human histiocytoma and TNF alpha antibody (clone TMDa1-1)

IHC testing of FFPE rat stomach and TNF alpha antibody (clone TMDa1-1)