TNF Antibody

• Catalog Number: orb1251879
• Category: Antibodies
• Description: TNF Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SPM543
• Tested applications: FC, IF, IHC
• Reactivity: Canine, Feline, Human, Mouse, Rabbit, Rat, Zebrafish
• Isotype: IgG1, kappa
• Immunogen: Amino acids 115-130 of the human protein was used as the immunogen for this anti-TNF alpha antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 2-4 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)The optimal dilution of the anti-TNF-alpha antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: TNF
• UniProt ID: P01375
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Tumor necrosis factor, Cachectin, TNF-alpha, Tumor
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 2-4 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)The optimal dilution of the anti-TNF-alpha antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Formalin-fixed, paraffin-embedded human polyostotic sclerosing histiocytosis (Erdheim Chester disease) stained with anti-TNF alpha antibody (SPM543).