TNF alpha Antibody

• Catalog Number: orb385905
• Category: Antibodies
• Description: Tumor Necrosis Factor Alpha (TNF alpha) is a cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective. Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line. [UniProt]
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgM, kappa
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Immunogen: Full length human recombinant protein was used as the immunogen for the TNF alpha antibody.
• UniProt ID: P01375
• Tested applications: FACS, IF, IHC-P
• Dilution range: Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT
• Application notes: Titering of the TNF alpha antibody may be required for optimal performance.
• Antibody Type: Primary Antibody
• Clone Number: TMDa1-1
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This TNF alpha antibody is available for research use only.
• Note: For research use only

Immunofluorescent staining of PFA-fixed human HepG2 cells with TNF-alpha antibody (green, clone TMDa1-1) and Reddot nuclear stain (red).

IHC testing of FFPE human histiocytoma and TNF alpha antibody (clone TMDa1-1). Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE rat stomach and TNF alpha antibody (clone TMDa1-1). Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

Flow cytometry testing of PFA-fixed human HepG2 cells with TNF-alpha antibody (clone TMDa1-1); Red = isotype control, Blue = TNF-alpha antibody.