TNF alpha Antibody

• Catalog Number: orb385905
• Category: Antibodies
• Description: Tumor Necrosis Factor Alpha (TNF alpha) is a cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective. Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line. [UniProt]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: TMDa1-1
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human, Rat
• Isotype: Mouse IgM, kappa
• Immunogen: Full length human recombinant protein was used as the immunogen for the TNF alpha antibody.
• Dilution range: Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT
• Purity: PEG precipitation
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This TNF alpha antibody is available for research use only.
• UniProt ID: P01375
• Storage: Store the TNF alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titering of the TNF alpha antibody may be required for optimal performance.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Human histiocytoma using TNF alpha antibody

Immunohistochemical staining of Rat stomach using TNF alpha antibody