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Catalog Number | orb1742580 |
---|---|
Category | Antibodies |
Description | SQSTM1 antibody, also known as ubiquitin-binding protein p62 antibody, detects the SQSTM1 protein that interacts with LC3 proteins to execute autophagosomal degradation. SQSTM1 is also reported to regulate the activation of NFκB1 downstream signaling in addition to having other cellular functions. |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FACS, ICC, IHC-P, IP, PLA, WB |
Reactivity | Bovine, Human, Insect, Mouse, Rat, Zebrafish |
Isotype | IgG |
Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human SQSTM1 / P62. The exact sequence is proprietary. |
Concentration | 0.39 mg/ml (Please refer to the vial label for the specific concentration.) |
Dilution range | WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. FACS: 1:50-1:200. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 48 |
Target | sequestosome 1 |
UniProt ID | Q13501 |
Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
Buffer/Preservatives | 0.025% ProClin 300. 1XPBS, 1% rAlbumin, 20% Glycerol (pH7) |
Alternative names | sequestosome 1 , A170 , DMRV , FTDALS3 , NADGP , O Read more... |
Note | For research use only |
Application notes | Positive control: NIH-3T3 , JC , BCL-1 , PC-12 , Rat2 , Huh7 ( 3μM Thapsigargin 12hr) , HepG2 |
Expiration Date | 12 months from date of receipt. |
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ICC, IF, IHC, WB | |
Hamster | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Animal, Bovine, Canine, Guinea pig, Human, Mouse, Rabbit, Rat, Yeast | |
Canine, Equine, Guinea pig, Human, Mouse, Rat, Yeast | |
Rabbit | |
Polyclonal | |
Unconjugated |
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