SQSTM1/p62 Antibody

• Catalog Number: orb507551
• Category: Antibodies
• Description: SQSTM1/p62 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC, WB
• Predicted Reactivity: Hamster
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human SQSTM1/p62 (69-96aa DEDGDLVAFSSDEELTMAMSYVKDDIFR), identical to the related mouse and rat sequences.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By HeatWestern blot, 0.1-0.5μg/ml, Human, Mouse, Monkey, RatImmunofluorescence, 2μg/ml, HumanImmunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse, RatImmunocytochemistry, 0.5-1μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 47687 MW
• UniProt ID: Q13501
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Sequestosome-1;EBI3-associated protein of 60 kDa;E
• Note: For research use only
• Application notes: WB: The detection limit for SQSTM1/p62 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of SQSTM1 using anti-SQSTM1 antibody.Lane 1:human HeLa cell; 2:human 293T cell; 3:human MCF-7 cell; 4:human Jurkat cell; 5:rat liver tissue; 6:rat brain tissue; 7:mouse liver tissue.

IF analysis of SQSTM1 using anti-SQSTM1 antibody.SQSTM1 was detected in immunocytochemical section of A431 cell.

IF analysis of SQSTM1/p62 using anti-SQSTM1/p62 antibody.SQSTM1/p62 was detected in paraffin-embedded section of human lung cancer tissues.

IHC analysis of SQSTM1 using anti-SQSTM1 antibody.SQSTM1 was detected in paraffin-embedded section of mouse small intestine tissues.

IHC analysis of SQSTM1 using anti-SQSTM1 antibody.SQSTM1 was detected in paraffin-embedded section of human intestinal cancer tissues.

IHC analysis of SQSTM1 using anti-SQSTM1 antibody.SQSTM1 was detected in paraffin-embedded section of rat small intestine tissue.

IHC analysis of SQSTM1 using anti-SQSTM1 antibody.SQSTM1 was detected in immunocytochemical section of A549 cell.

IHC analysis of SQSTM1 using anti-SQSTM1 antibody.SQSTM1 was detected in frozen section of human placenta tissue.