SQSTM1 Antibody

• Catalog Number: orb1261282
• Category: Antibodies
• Description: SQSTM1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-440 of human SQSTM1 / p62 (NP_001135770.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 62kDa
• Target: SQSTM1
• UniProt ID: Q13501
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: SQSTM1 Antibody: p60, p62, A170, OSIL, PDB3, ZIP3,
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using SQSTM1 antibody (orb1261282) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded human tonsil using SQSTM1/p62 antibody (orb1261282) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon carcinoma using SQSTM1/p62 antibody (orb1261282) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human tonsil using SQSTM1/p62 antibody (orb1261282) at dilution of 1:150 (40x lens).

Immunohistochemistry of paraffin-embedded human colon carcinoma using SQSTM1/p62 antibody (orb1261282) at dilution of 1:150 (40x lens).

Immunofluorescence analysis of HeLa cells using SQSTM1/p62 antibody (orb1261282) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using SQSTM1/p62 antibody (orb1261282) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of C6 cells using 3 ug SQSTM1/p62 antibody (orb1261282). Western blot was performed from the immunoprecipitate using SQSTM1/p62 antibody (orb1261282) at a dilution of 1:1000.