Sequestosome-1 Antibody

• Catalog Number: orb1525872
• Category: Antibodies
• Description: Sequestosome-1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 325 and 375
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Sequestosome-1
• UniProt ID: Q13501
• NCBI: NP_003891.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: p60; ZIP3; ubiquitin-binding protein p62; sequesto
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse Sequestosome-1 by western blot. Samples: Whole cell lysate from HeLa (5, 15, and 50 µg) , HEK293T (T; 50 µg) , and mouse NIH 3T3 (M; 50 µg) cells. Antibody: Affinity purified rabbit anti-Sequestosome-1 antibody orb1525872 used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human Sequestosome-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Sequestosome-1 antibody orb1525872 used for IP at 3 µg/mg lysate.

Detection of human Sequestosome-1 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Sequestosome-1 (Cat. No. orb1525872) used at a dilution of 1: 1, 000 (1µg/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate