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Catalog Number | orb1230157 |
---|---|
Category | Proteins |
Description | RANKL Recombinant Protein |
Tag | Fc Tag |
Concentration | 0.1mg/ml after reconstitution. |
Form/Appearance | Lyophilized |
Purity | > 95% (SDS-PAGE). Endotoxin level is less than 0.01EU/ μg purified protein (LAL test; Lonza). |
Target | Tnfsf11 |
UniProt ID | O14763 |
Protein Sequence | Mouse RANKL (aa 157-316) is fused at the N-terminus to the Fc portion of human IgG1. |
Source | HEK293 cells |
Biological Activity | Induces osteoclastogenesis of bone marrow-derived macrophages. |
NCBI | O35235 |
Storage | Stable for at least 6 months after receipt when stored at -20˚°C. |
Buffer/Preservatives | Contains PBS. Reconstitute with 100 μl sterile water. |
Alternative names | TRANCE, ODF, OPGL, TNFSF11, CD254 Read more... |
Note | For research use only |
Application notes | This recombinant proteins is for research use only. |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 48.4 kDa after removal of the signal peptide.The apparent molecular mass of hFc-TNFSF11 is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
Greater than 90% as determined by SDS-PAGE. | |
22.4 kDa | |
E.coli |
ELISA, WB | |
> 95% as determined by SDS-PAGE. | |
47.4 kDa | |
HEK293 cells |
ELISA, WB | |
> 90% as determined by SDS-PAGE. | |
46.9 kDa | |
HEK293 cells |
Greater than 90% as determined by SDS-PAGE. | |
43.9 kDa | |
E.coli |