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Human RANKL protein

SKU: orb594839
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ActiveBiologically Active

Description

This Human RANKL protein spans the amino acid sequence from region 140-317aa. Purity: Greater than 90% as determined by SDS-PAGE.

Images & Validation

Application Notes
Partial

Key Properties

SourceE.coli
Biological OriginHomo sapiens (Human)
Biological Activity①Loaded Recombinant Human OPG-Fc on Pro A Biosensor, can bind Human RANKL with an affinity constant of 1.83 pM as determined in BLI assay. ②Loaded Human RANK-His on HIS1K Biosensor, can bind Human RANK L with an affinity constant of <1 pM as determined in BLI assay.
TagTag-Free
Molecular Weight22.4 kDa
Expression Region140-317aa
Protein LengthPartial
Protein SequenceIRAEKAMVDGSWLDLAKRSKLEAQPFAHLTINATDIPSGSHKVSLSSWYHDRGWAKISNMTFSNGKLIVNQDGFYYLYANICFRHHETSGDLATEYLQLMVYVTKTSIKIPSSHTLMKGGSTKYWSGNSEFHFYSINVGGFFKLRSGEEISIEVSNPSLLDPDQDATYFGAFKVRDID
PurityGreater than 90% as determined by SDS-PAGE.
EndotoxinsLess than 1.0 EU/µg as determined by LAL method.

Storage & Handling

StorageThe shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Form/AppearanceLyophilized powder
Buffer/PreservativesLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 150 mM NaCl, pH 8.0
DisclaimerFor research use only

Alternative Names

CD254; ODF; OPGL; RANK L; TNFSF11; CD254; Osteoclast differentiation factor; Receptor activator of nuclear factor kappa-B ligand; tumor necrosis factor ligand superfamily member 11

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Human RANKL protein

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

UniProt Details

No UniProt data available

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Protocol Information

Human RANKL protein (orb594839)

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Available Sizes

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10 μg
$ 340.00
50 μg
$ 850.00
500 μg
$ 2,780.00
1 mg
$ 3,940.00
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