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Catalog Number | orb594839 |
---|---|
Category | Proteins |
Description | Recombinant Human Tumor necrosis factor ligand superfamily member 11(TNFSF11),partial (Active) |
Tag | Tag free |
Form/Appearance | Lyophilized powder |
Purity | Greater than 90% as determined by SDS-PAGE. |
MW | 22.4 kDa |
UniProt ID | O14788 |
Protein Sequence | IRAEKAMVDGSWLDLAKRSKLEAQPFAHLTINATDIPSGSHKVSLSSWYHDRGWAKISNMTFSNGKLIVNQDGFYYLYANICFRHHETSGDLATEYLQLMVYVTKTSIKIPSSHTLMKGGSTKYWSGNSEFHFYSINVGGFFKLRSGEEISIEVSNPSLLDPDQDATYFGAFKVRDID |
Protein Length | Partial |
Source | E.coli |
Expression System | 140-317aa |
Biological Activity | The ED50 as determined by its ability to binding SF11A used funtional ELISA is less than 10 ug/ml. |
Expression Region | 140-317aa |
Endotoxins | Less than 1.0 EU/µg as determined by LAL method. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 150 mM NaCl, pH 8.0 |
Alternative names | CD254; ODF; OPGL; RANK L; TNFSF11; CD254; Osteocla Read more... |
Note | For research use only |
Application notes | Partial |
Expiration Date | 6 months from date of receipt. |
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Unconjugated | |
95% | |
20.5 kDa | |
Human TNFSF11 Protein, premium grade (orb1149290) is expressed from human 293 cells (HEK293). It contains AA Gly 64 - Asp 245 (Accession # AAC51762.1). |
Unconjugated | |
95% | |
44.4 kDa | |
Human Osteoprotegerin, His Tag (orb257888) is expressed from human 293 cells (HEK293). It contains AA Glu 22 - Leu 401 (Accession # NP_002537). |
Unconjugated | |
92% | |
70.2 kDa | |
Human Osteoprotegerin, Fc Tag (orb257890) is expressed from human 293 cells (HEK293). It contains AA Glu 22 - Leu 401 (Accession # NP_002537). |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 48.4 kDa after removal of the signal peptide.The apparent molecular mass of hFc-TNFSF11 is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |
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