TNFSF11 / RANKL / CD254 Recombinant Protein

• Catalog Number: orb1231211
• Category: Proteins
• Description: TNFSF11 / RANKL / CD254 Recombinant Protein
• Tested applications: ELISA, WB
• Tag: Fc Tag
• Form/Appearance: Lyophilized
• Purity: > 90% as determined by SDS-PAGE.
• MW: 46.9 kDa
• Target: TNFSF11
• UniProt ID: O14788
• Protein Sequence: Gly 64 - Asp 245
• Source: HEK293 cells
• NCBI: AAC51762.1
• Storage: Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: PBS, pH 7.4
• Alternative names: RANKL,CD254,TRANCE,OPGL,ODF
• Note: For research use only
• Application notes: This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 46.9 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Human TNFSF11, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Immobilized Human RANK, Mouse IgG2a Fc Tag, low endotoxin at 0.5 ug/ml (100 uL/well) can bind Human TNFSF11, Fc Tag with a linear range of 0.1-3 ng/mL.

Immobilized Human OPG, His Tag at 10 ug/ml (100 uL/well) can bind Human TNFSF11, Fc Tag with a linear range of 0.3-2 ng/mL.