Rabbit anti-Phospho KAP-1 (S824) Antibody

• Catalog Number: orb1529674
• Category: Antibodies
• Description: KAP-1, Phospho (S824) Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Surrounding serine 824
• Antibody Type: Primary Antibody
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:200 - 1:1,000; ICC-IF - 1:200 - 1:1,000
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: KAP-1, Phospho (S824)
• UniProt ID: Q13263
• NCBI: NP_005753.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: KRAB [Kruppel-associated box domain]-associated pr
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human phospho KAP-1 (S824) by western blot.Samples: Whole cell lysate from HEK293T cells treated with 100 µM etoposide (+) or mock treated (-). Antibody: Affinity purified rabbit anti-phospho KAP-1 (S824) antibody at 0.04 µg/ml.

Detection of mouse phospho KAP-1 (S824) by western blot.Samples: Whole cell lysate from NIH 3T3 cells treated with 100 µM etoposide (+) or mock treated (-). Antibody: Affinity purified rabbit anti-phospho KAP-1 (S824) antibody at 0.04 µg/ml.

Detection of human Phospho KAP-1 (S824) by IHC.Samples: FFPE serial sections of prostate carcinoma with MOCK CIP treatment (left) and CIP treatment (right). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) at a dilution of 1:200.

Detection of human phospho KAP-1 (S824) by WB of immunoprecipitates.Samples: HEK293T cells. Antibodies: Affinity purified rabbit anti-phospho KAP-1 (S824) antibody and rabbit anti-KAP-1 recombinant monoclonal antibody.

Detection of human Phospho KAP-1 (S824) by ICC.Samples: HeLa cells with MOCK CIP treatment (left) and with CIP treatment (right). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) used at a dilution of 1:200.

Detection of human Phospho KAP-1 (S824) by ICC.Samples: HeLa cells. Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) used at a dilution of 1:200. Detection: Red fluorescent anti-rabbit IgG-DyLight® 594 conjugated.