Rabbit anti-Phospho KAP-1 (S824) Antibody

• Catalog Number: orb1523531
• Category: Antibodies
• Description: KAP-1, Phospho (S824) Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Mouse
• Immunogen: Surrounding serine 824
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: KAP-1, Phospho (S824)
• UniProt ID: Q62318
• NCBI: NP_035718.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: E3 SUMO-protein ligase TRIM28; Tif1beta; tripartit
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse Phospho KAP-1 (S824) by western blot. Samples: Whole cell lysate (10 µg) from BeWo and AlphaTC1 Clone 9 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) antibody (orb1523531) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Detection of mouse Phospho KAP-1 (S824) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from TCMK cells treated with etoposide (+) and/or Lambda PP (+) or mock treated (-). Antibodies: Affinity purified rabbit anti- KAP-1 antibody used for IP at 6 µg per reaction.