Protein A antibody

• Catalog Number: orb420286
• Category: Antibodies
• Description: Protein A antibody
• Species/Host: Gallus
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, WB
• Isotype: IgY
• Immunogen: This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with Protein A [Staphylococcus aureus].
• Concentration: 1 mg/ml
• Dilution range: ELISA: 1:500 - 1:2,000, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This Anti-Protein A Antibody is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Protein A [Staphylococcus aureus] and anti-Chicken Serum. Cross reactivity against Protein A from other sources is unknown.
• Conjugation: Unconjugated
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: chicken anti-Protein A Antibody, chicken anti Prot
• Note: For research use only
• Application notes: Anti-PROTEIN A (CHICKEN) Antibody has been tested by western blotting and suitable for ELISA. Although not tested, this antibody may also be useful for neutralization assays, immunohistochemistry, flow Cytometry, immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring high titer and specificity.
• Expiration Date: 12 months from date of receipt.