Protein A antibody (FITC)

• Catalog Number: orb344224
• Category: Antibodies
• Description: Protein A antibody (FITC)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: DOT, FC, WB
• Isotype: IgG
• Immunogen: Protein A (Staphylococcus aureus)
• Concentration: 1.0 mg/mL
• Dilution range: FC: User Optimized
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Protein A Fluorescein Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Goat Serum, and purified and partially purified Protein A.
• Conjugation: FITC
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: goat anti-Protein A Antibody, FITC Conjugated goat
• Note: For research use only
• Application notes: Anti-Protein A has been tested in dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Protein A Reduced (Lane1), Protein A Reduced (Lane2), Protein A Non-Reduced (Lane3), Protein A Non-Reduced (Lane4), Load: Lane 1 and 3 - 1.0 ug per lane, Lane 2 and 4 - 0.25 ug per lane using Protein A antibody (FITC)