Nurr1 antibody (FITC)

• Catalog Number: orb464231
• Category: Tools
• Description: Nurr1 antibody (FITC)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF
• Predicted Reactivity: Bovine, Canine, Equine, Mouse, Porcine, Sheep
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Nurr1
• Concentration: 1mg/ml
• Dilution range: Flow-Cyt=1μg/Test,IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: FITC
• Target: Nurr1
• UniProt ID: P43354
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: Nur-ralated factor 1; Nuclear receptor-related fac
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Zhang, Wencan et al. Steroid nuclear receptor coactivator 2 controls immune tolerance by promoting induced Treg differentiation via up-regulating Nr4a2 Sci Adv, 8, (2022)

PubMed: 35704583

Applications

FC

Reactivity

Mouse

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-Nurr1 antibody (orb464231-FITC) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Protocol The cells were fixed with 4% PFA (10min at room temperature)

Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-Nurr1 antibody (orb464231-FITC) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Protocol The cells were fixed with 4% PFA (10min at room temperature)

Blank control:U-87MG. Primary Antibody (green line): Rabbit Anti-Nurr1 antibody (orb464231-FITC) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Protocol The cells were fixed with 4% PFA (10min at room temperature)

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-Nurr1 antibody (orb464231-FITC) Dilution: 1ug/Test; Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -2