CD135 antibody (FITC)

• Catalog Number: orb154474
• Category: Antibodies
• Description: Mouse monoclonal antibody conjugated to FITC which recognizes CD135 that belongs to the protein kinase superfamily, Tyr protein kinase family. It expressed in bone marrow cells. Receptor for the FL cytokine. Has a tyrosine-protein kinase activity. CD135 plays a role in cell survival, proliferation, and differentiation. CD135 is also important for lymphocyte (B cell and T cell) development.
• Clonality: Monoclonal
• Clone Number: BV10A4
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: BV-173 leukemic cell line
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Purity: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: FITC
• Target: CD135
• Entrez: 2322
• UniProt ID: P36888
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-FLT3 antibody, anti-FLK2 antibody, anti-STK1
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Separation of REH cells stained using anti-human CD135 (BV10A4) FITC antibody (4 μl reagent per million cells in 100 μl of cell suspension, red-filled) from REH cells unstained by primary antibody (black-dashed) in flow cytometry analysis (surface staining).

Flow cytometry surface staining pattern of REH cellular suspension stained using anti-human CD135 (BV10A4) FITC antibody (4 μl reagent per million cells in 100 μl of cell suspension).