HCN4 antibody

• Catalog Number: orb148502
• Category: Antibodies
• Description: Mouse monoclonal to HCN4 (FITC). Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand- gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels. Specifically, hyperpolarization-activated cation channels of the HCN gene family contribute to spontaneous rhythmic activity in both the heart and brain.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: N114/10 (Formerly sold as S114-10)
• Tested applications: AM, FACS, ICC, IHC, WB
• Reactivity: Human, Mouse, Rabbit, Rat
• Isotype: IgG1
• Immunogen: Fusion protein amino acids 1019-1198 (c-terminus) of rat HCN4
• Concentration:

  1 mg/ml

• Dilution range: WB: 1:1000, IHC: 1:1000, IF/ICC: 1:100
• Purity: Protein G Purified
• Conjugation: FITC
• MW: 130kDa
• Target: HCN4
• Entrez: 59266
• UniProt ID: Q9JKA7
• NCBI: NP_067690.1
• Storage: -20ºC, Conjugated antibodies should be stored at 4ºC
• Buffer/Preservatives: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Alternative names: Hyperpolarization activated cyclic nucleotide gate
• Note: For research use only
• Application notes: 1 µg/ml of SMC-320 was sufficient for detection of HCN4 in 10 µg of COS cell lysate transiently transfected with HCN4 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry analysis human hippocampus using HCN4 antibody.

Immunofluorescence analysis Human Neuroblastoma cells (SH-SY5Y) using HCN4 antibody.

Immunohistochemistry analysis Mouse frozen brain section using HCN4 antibody.

Western Blot analysis of Human T-HEK cell lysate using HCN4 antibody.