Occludin Antibody

• Catalog Number: orb639560
• Category: Antibodies
• Description: Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of Occludin. Expression of Occludin is upregulated by polyunsaturated fatty acids, increasing trans-endothelial cell resistance and reducing cellular permeability to large molecules. The level of Occludin varies greatly depending on tissue; in brain tissue, Occludin is highly expressed at cell-cell contact sites. Non-neural tissues show lower expression and discontinuous distribution. Up-regulation of epithelial Occludin may play a role in enhancing paracellular permeability and be related to the damage to the tight junction.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: OCLN/2181
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A recombinant human partial protein (amino acids 282-415) was used as the immunogen for the Occludin antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Occludin antibody is available for research use only.
• UniProt ID: Q16625
• Storage: Store the Occludin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human thyroid carinoma with Occludin antibody (clone OCLN/2181). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE human renal cell carinoma with Occludin antibody (clone OCLN/2181). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Western blot testing of human MCF7 cell lysate with Occludin antibody. Predicted molecular weight ~59 kDa.

SDS-PAGE analysis of purified, BSA-free Occludin antibody (clone OCLN/2181) as confirmation of integrity and purity.