Nestin (NES) antibody

• Catalog Number: orb1294409
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Nestin antibody (NES)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rabbit
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the N-terminus region.
• Dilution range: Western Blot 1:500-1:1000 Immunofluorescence 1:200-1:500 Immunohistochemistry (Paraffin) 1:100-1:200
• Conjugation: Unconjugated
• Target: Nestin / NES
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence:cells were stained with anti-Nestin antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Nestin with orb1294409 at 1:100.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Nestin with orb1294409 at 1:100.

Western blot analysis of Neuron 2A using Nestin antibody at 1:1000 dilution.

Western blot analysis using Anti-Nestin antibody at 1:1000 dilution, lysates at 30 μg per lane.