Nestin Antibody

• Catalog Number: orb334607
• Category: Antibodies
• Description: Nestin Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Nestin (252-290aa ERLRATEKFQLAVEALEQEKQGLQSQIAQVLEGRQQLAH), different from the related mouse sequence by four amino acids, and from the related rat sequence by five amino acids.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By HeatImmunohistochemistry (Frozen Section), 0.5-1μg/ml Western blot, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 177439 MW
• UniProt ID: P48681
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Nestin;NES;Nbla00170;
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of Nestin using anti-Nestin antibody.Lane 1:HELA cell;2:HEPG2 cell.

IHC analysis of Nestin using anti-Nestin antibody. Nestin was detected in a paraffin-embedded section of rat kidney tissue.

IHC analysis of Nestin using anti-Nestin antibody. Nestin was detected in a paraffin-embedded section of human melanoma tissue.

IHC analysis of Nestin using anti-Nestin antibody.Nestin was detected in frozen section of mouse kidney tissue.

IHC analysis of Nestin using anti-Nestin antibody.Nestin was detected in frozen section of rat kidney tissue.

IHC analysis of Nestin using anti-Nestin antibody. Nestin was detected in a paraffin-embedded section of rat kidney tissue.