Nestin (S1409) antibody

• Catalog Number: orb1431842
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Nestin (S1409).
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB16253
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: Synthetic Peptide
• Dilution range: IF: 1:200, IF: 1:25, WB: 1:2000, WB: 1:2000, IHC-P: 1:25, IHC-P: 1:25, IHC-P: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 177439
• Target: NES
• UniProt ID: P48681
• NCBI: NP_006608.1
• Alternative names: Nestin, NES
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Fluorescent confocal image of SH-SY5Y cells stained with Nestin (S1409) antibody at 1:200 dilution.Secondary antibody was Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green).Nuclei were counterstained with Hoechst 33342 (blue).

Immunofluorescent analysis of U-2 OS (human bone osteosarcoma cell line) cells labeling Nestin with orb1431842 at 1:25 dilution. Dylight® 488-conjugated goat anti-rabbit IgG(green);Dylight® 554 Phalloidin (red);Nuclear counter stain is DAPI (blue).

Western blot:All lanes:Anti-Nestin Antibody (S1409) at 1:2000 dilution.Lane 1:U-251 MG;Lane 2:SH-SY5Y.

Western blot:Anti-Nestin Antibody (S1409) at 1:2000 dilution + Hela.Secondary antibody was Goat Anti-Rabbit IgG, (H+L).

Orb1431842 staining Nestin in Human heart tissue sections by immunohistochemistry at 1:25 dilution.A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Orb1431842 staining Nestin in human brain tissue sections by immunohistochemistry at 1:25 dilution.A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Orb1431842 staining Nestin in human cerebellum tissue sections by immunohistochemistry at 1:25 dilution.A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.