Nestin antibody

• Catalog Number: orb11108
• Category: Tools
• Description: Nestin antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IHC-P
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Nestin (801-900/1621 aa)
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, ICC=1:100, Flow-Cyt=1ug/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 178 kDa
• Target: Nestin
• UniProt ID: P48681
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: ESTM 46; FLJ 21841; FLJ21841; Intermediate filamen
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraffin-embedded glioma of human brain tissue fixed with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (37 degrees for 20 min.). Nestin antibody at 1:200 dilution with overnight incubation at 4 degre