MCP-1/MCAF Antibody

• Catalog Number: orb1272402
• Category: Antibodies
• Description: MCP-1/MCAF Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, NeA, WB
• Reactivity: Rat
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat MCP-1/MCAF (Macrophage/Monocyte chemotactic protein-1).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: Ccl2
• UniProt ID: P14844
• NCBI: P14844
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MCP-1, Scya2, Sigje, Je, Mcp1, C-C motif chemokine
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

To detect Rat MCP-1 (MCAF) by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Rat MCP-1 (MCAF) (orb1272401) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat MCP-1 (MCAF).

To detect Rat MCP-1 (MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat MCP-1 (MCAF) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

To detect Rat MCP-1 (MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat MCP-1 (MCAF) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained colchicine injected rat brain (including the caudate putamen) tissue. The primary antibody was incubated at 1.0 ug/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.