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MCP-1/MCAF Antibody (biotin)
Catalog Number: orb1272403
| Catalog Number | orb1272403 |
|---|---|
| Category | Antibodies |
| Description | MCP-1/MCAF Antibody (biotin) |
| Target | CCL2 |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Conjugation | Biotin |
| Reactivity | Human |
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMCP-1(MCAF) (human Macrophage Chemoattractant Protein-1). |
| UniProt ID | P13500 |
| Tested applications | ELISA, WB |
| Application notes | ELISA:Sandwich:To detect hMCP-1/MCAF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human MCP-1/MCAF (XP-5218) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMCP-1/MCAF.Western Blot:To detect hMCP-1(MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-1(MCAF) is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
| Antibody Type | Primary Antibody |
| Storage | MCP-1/MCAF antibody is stable for at least 2 years from date of receipt at -20˚°C. The reconstituted antibody is stable for at least two weeks at 2-8˚°C. Frozen aliquots are stable for at least 6 months when stored at -20˚°C. Avoid repeated freeze-thaw cycles. |
| Alternative names | HC11, MCAF, MCP1, MCP-1, SCYA2, GDCF-2, SMC-CF, HS Read more... |
| Note | For research use only |
| NCBI | P13500 |
To detect hMCP-1/MCAF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Anti-Human MCP-1/MCAF (orb1272404) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hMCP-1/MCAF.
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