MBP Antibody / Myelin Basic Protein

• Catalog Number: orb699550
• Category: Antibodies
• Description: Myelin basic protein (MBP) is the second most abundant protein in central nervous system (CNS) myelin: it comprises 30% of the total protein and about 10% of the dry weight of myelin. It is the only structural protein found so far to be essential for formation of CNS myelin, and has been called the executive molecule of myelin . MBP can interact with a number of polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. It may act as a membrane actin-binding protein, which might allow it to participate in transmission of extracellular signals to the cytoskeleton in oligodendrocytes and tight junctions in myelin. MBP may be applicable as a marker for oligodendrogliomas.
• Clonality: Recombinant
• Species/Host: Mouse
• Isotype: Mouse IgG2a, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Purity: Protein G affinity chromatography
• Immunogen: A portion of amino acids 150-250 from the human protein was used as the immunogen for the recombinant MBP antibody.
• UniProt ID: P02686
• Tested applications: IHC-P, WB
• Dilution range: Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Application notes: Optimal dilution of the recombinant MBP antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: rMBP/4288
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Store the recombinant MBP antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Hazard Information: This recombinant MBP antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human cerebellum with recombinant MBP antibody (clone rMBP/4288). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human cerebellum with recombinant MBP antibody (clone rMBP/4288). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human brain lysate with recombinant MBP antibody (clone rMBP/4288). Isoforms may be visualized from 20~37 kDa.

SDS-PAGE analysis of purified, BSA-free recombinant MBP antibody (clone rMBP/4288) as confirmation of integrity and purity.