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MBP Antibody / Myelin Basic Protein

Catalog Number: orb2644745

DispatchUsually dispatched within 5-10 working days
$ 780.00
Catalog Numberorb2644745
CategoryAntibodies
DescriptionMyelin basic protein (MBP) is the second most abundant protein in central nervous system (CNS) myelin: it comprises 30% of the total protein and about 10% of the dry weight of myelin. It is the only structural protein found so far to be essential for formation of CNS myelin, and has been called the executive molecule of myelin. MBP can interact with a number of polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. It may act as a membrane actin-binding protein, which might allow it to participate in transmission of extracellular signals to the cytoskeleton in oligodendrocytes and tight junctions in myelin. MBP may be applicable as a marker for oligodendrogliomas. MBP/4272 recognizes an epitope in the 119-131 region of MBP, useful in clinical diagnosis to detect MBP levels in human, rat and cow MBP.
Species/HostMouse
ClonalityMonoclonal
Clone NumberMBP/4272
Tested applicationsIHC-P, WB
ReactivityHuman
IsotypeMouse IgG1
ImmunogenAmino acids GAEGQRPGFGYGG were used as the immunogen for the MBP antibody.
Antibody TypePrimary Antibody
Dilution rangeWestern blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Hazard InformationThis MBP antibody is available for research use only.
UniProt IDP02686
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
NoteFor research use only
Application notesOptimal dilution of the MBP antibody should be determined by the researcher.
Expiration Date12 months from date of receipt.
MBP Antibody / Myelin Basic Protein

SDS-PAGE analysis of purified, BSA-free MBP antibody as confirmation of integrity and purity.

MBP Antibody / Myelin Basic Protein

Western blot testing of human U-87 MG cell lysate with MBP antibody. Isoforms may be visualized from 20~37 kDa.

MBP Antibody / Myelin Basic Protein

IHC staining of FFPE human brain with MBP antibody. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

MBP Antibody / Myelin Basic Protein

IHC staining of FFPE human brain with MBP antibody. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

MBP Antibody / Myelin Basic Protein

Western blot testing of human brain lysate with MBP antibody. Isoforms may be visualized from 20~37 kDa.

  • MBP Antibody / Myelin Basic Protein [orb699742]

    IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg
  • MBP Antibody / Myelin Basic Protein [orb699549]

    IHC-P

    Human

    Rabbit

    Recombinant

    Unconjugated

    20 μg
  • MBP Antibody / Myelin Basic Protein [orb699550]

    IHC-P,  WB

    Human

    Mouse

    Recombinant

    Unconjugated

    20 μg
  • MBP Antibody / Myelin Basic Protein [orb699741]

    IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg
  • MBP Antibody / Myelin Basic Protein [orb699743]

    IHC-P,  WB

    Human, Mouse

    Rat

    Monoclonal

    Unconjugated

    20 μg