LIGHT antibody

• Catalog Number: orb20341
• Category: Antibodies
• Description: Goat polyclonal to LIGHT
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Human
• Dilution range: ELISA: 1:64000, WB: 0.03-0.1 μg/ml, IHC-P: 10 μg/ml
• Conjugation: Unconjugated
• MW: 26.4; 22.4
• Target: LIGHT / CD258
• Entrez: 8740
• Protein Sequence: HEVNPAAHLTGANS
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti TNFSF14 antibody, anti tumor necrosis factor
• Note: For research use only
• Application notes: This antibody is expected to recognize both reported isoforms (NP_003798.2; NP_742011.1).ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 22kDa band observed in Human Brain (Cerebellum and Frontal Cortex) lysates (calculated MW of 22.4kDa according to NP_742011.1). Recommended concentration: 1-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb20341 (1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

WB analysis of Human Cerebellum (A) and Frontal Cortex (B) lysate using LIGHT antibody

Immunofluorescence analysis of HeLa cells using LIGHT antibody