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Catalog Number | orb402263 |
---|---|
Category | Antibodies |
Description | Integrin alpha 5/ITGA5 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, ICC, IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E. coli-derived human Integrin alpha 5 recombinant protein (Position: F42-A263). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunofluorescence, 2μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 150 kDa |
UniProt ID | P08648 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Integrin alpha-5; CD49 antigen-like family member Read more... |
Note | For research use only |
Application notes | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of HT-1080 cells using anti-Integrin alpha 5 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
Flow Cytometry analysis of A431 cells using anti-Integrin alpha 5 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of Integrin alpha 5 using anti-Integrin alpha 5 antibody.Lane 1:human HeLa Cell;2:human HepG2 Cell;3:rat liver Tissue;4:mouse liver Tissue.
IF analysis of Integrin alpha 5 using anti-Integrin alpha 5 antibody.Integrin alpha 5 was detected in paraffin-embedded section of human colon cancer tissues.
IF analysis of Integrin alpha 5 using anti-Integrin alpha 5 antibody.Integrin alpha 5 was detected in paraffin-embedded section of human placenta tissues.
IF analysis of Integrin alpha 5 using anti-Integrin alpha 5 antibody. Integrin alpha 5 was detected in immunocytochemical section of HeLa cells.
IF analysis of Integrin alpha 5 using anti-Integrin alpha 5 antibody. Integrin alpha 5 was detected in immunocytochemical section of U20S cells.
IHC analysis of Integrin alpha 5 using anti-Integrin alpha 5 antibody.Integrin alpha 5 was detected in paraffin-embedded section of human colon cancer tissue.
IHC analysis of Integrin alpha 5 using anti-Integrin alpha 5 antibody.Integrin alpha 5 was detected in paraffin-embedded section of human lung cancer tissue.
IHC analysis of Integrin alpha 5 using anti-Integrin alpha 5 antibody.Integrin alpha 5 was detected in paraffin-embedded section of rat cardiac muscle tissue.
IHC analysis of Integrin alpha 5 using anti-Integrin alpha 5 antibody.Integrin alpha 5 was detected in paraffin-embedded section of human placenta tissue.
IHC analysis of Integrin alpha 5 using anti-Integrin alpha 5 antibody.Integrin alpha 5 was detected in paraffin-embedded section of mouse cardiac muscle tissue.
FC, WB | |
Bovine, Canine, Equine, Mouse, Porcine, Sheep |
WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
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