Cleaved LC3 antibody

• Catalog Number: orb33336
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Cleaved LC3
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB38908
• Tested applications: ICC, IF, IHC-P, WB
• Predicted Reactivity: Rat, Zebrafish
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Immunogen: Synthetic Peptide
• Dilution range: IF/ICC: 1:25, IHC-P: 1:10-50, WB: 1:1000, IF/ICC-1:10-50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Target: This Cleaved LC3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-120 amino acids from human Cleaved LC3A or LC3B.
• UniProt ID: Q9H492, Q9GZQ8
• NCBI: NP_115903.1, NP_852610.1
• Alternative names: anti ATG8F antibody, anti Autophagy-related protei
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Miyamoto, Morikazu et al. Inhibition of autophagy protein LC3A as a therapeutic target in ovarian clear cell carcinomas J Gynecol Oncol, 28, e33 (2017)

PubMed: 28382796

Bingel, Corinna et al. Three-dimensional tumor cell growth stimulates autophagic flux and recapitulates chemotherapy resistance Cell Death Dis, 8, e3013 (2017)

PubMed: 28837150

WB analysis of A431 cell line using Cleaved LC3 antibody

WB analysis of A431 cell line using Cleaved LC3 antibody

WB analysis of A431 cell line using Cleaved LC3 antibody

WB analysis of NIH/3T3 cells using Cleaved LC3 antibody

Confocal immunofluorescense analysis of U251 cells lysate using Cleaved LC3A antibody

Confocal immunofluorescense analysis of U251 cells lysate using Cleaved LC3A antibody

Confocal immunofluorescense analysis of 5Y cells lysate using Cleaved LC3A antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded human cancer tissue using Cleaved LC3A antibody

WB analysis of NIH/3T3 cells using Cleaved LC3 antibody