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Catalog Number | orb20535 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to LARGE |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA |
Reactivity | Bovine, Human, Mouse, Rat |
Dilution range | ELISA: 1:32000, WB: 0.5 μg/ml |
Conjugation | Unconjugated |
MW | 88.1 |
Target | LARGE (N-terminus) |
Entrez | 9215 |
Protein Sequence | RYTASSQRERESLE |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti acetylglucosaminyltransferase-like 1A antibod Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 125kDa band in Human Kidney, Pancreas and Placenta lysates after 0.5 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 88.1kDa according to NP_004728.1. The 125kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of Human Kidney lysate using LARGE antibody
ELISA, IF, IHC, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human, Monkey, Mouse | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
Unconjugated | |
90% | |
101.9 kDa | |
SARS-CoV-2 S1 protein, Mouse IgG2a Fc Tag (orb640170) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Arg 685 (Accession # QHD43416.1). |
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