Calpain 2 CAPN2 Antibody (monoclonal, 8I6)

• Catalog Number: orb507561
• Category: Antibodies
• Description: Calpain 2 CAPN2 Antibody (monoclonal, 8I6)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 8I6
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Monkey, Mouse
• Isotype: Mouse IgG2a
• Immunogen: E. coli-derived human Calpain 2 recombinant protein (Position: R500-L700). Human Calpain 2 shares 93.5% and 92.5% amino acid (aa) sequence identity with mouse and rat Calpain 2, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 80 kDa
• UniProt ID: P17655
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Calpain-2 catalytic subunit; Calcium-activated neu
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A431 cells using anti-Calpain 2 antibody (Blue line).Isotype control antibody (Green line) was mouse IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of U87 cells using anti-Calpain 2 antibody (Blue line).Isotype control antibody (Green line) was mouse IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Calpain 2 using anti-Calpain 2 antibody.Lane 1:A549 cell; 2:U20S cell; 3:COS-7 cell; 4:K562 cell; 5:HELA cell; 6:HEPG2 cell.

IF analysis of Calpain 2 using anti-Calpain 2 antibody. Calpain 2 was detected in immunocytochemical section of A549 cells.

IF analysis of Calpain 2 using anti-Calpain 2 antibody. Calpain 2 was detected in immunocytochemical section of U251 cells.

IHC analysis of Calpain 2 using anti-Calpain 2 antibody. Calpain 2 was detected in paraffin-embedded section of human intestinal cancer tissue.

IHC analysis of Calpain 2 using anti-Calpain 2 antibody. Calpain 2 was detected in paraffin-embedded section of human mammary cancer tissue.

IHC analysis of Calpain 2 using anti-Calpain 2 antibody. Calpain 2 was detected in paraffin-embedded section of mouse lung tissue.