Casp12 Antibody

• Catalog Number: orb1239263
• Category: Antibodies
• Description: Casp12 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Caspase-12 antibody was raised against a synthetic peptide corresponding to 16 amino acids within the large cleavage product of murine caspase-12.The immunogen is located within amino acids 170 - 220 of Caspase-12.
• Concentration: 1 mg/mL
• Dilution range: Caspase-12 (large) antibody can be used for the detection of both the propeptide and cleaved forms of caspase-12 by Western blot at 0.25 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human, mouse, and rat samples; Immunohistochemistry in human, mouse, and rat samples and Immunofluorescence in human, mouse, and rat samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 46 kDa Observed: 46 kDa
• Target: Casp12
• UniProt ID: O08736
• NCBI: CAA73532
• Storage: Caspase-12 (Large) antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Caspase-12 Antibody is supplied in PBS containing 0.02% sodium azide. (Large)
• Alternative names: Caspase-12 (Large) Antibody: Caspase-12, CASP-12
• Note: For research use only
• Application notes: Caspase-12 (large) antibody can be used for the detection of both the propeptide and cleaved forms of caspase-12 by Western blot at 0.25 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human, mouse, and rat samples; Immunohistochemistry in human, mouse, and rat samples and Immunofluorescence in human, mouse, and rat samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of human heart tissue using caspase-12 antibody at 2 µg/mL.

Immunofluorescence of Caspase-12 in Human Heart tissue with Caspase-12 antibody at 10 µg/mL.

Immunohistochemistry of Caspase-12 in rat heart tissue with Caspase-12 antibody at 2.5 µg/ml.

Immunofluorescence of Caspase-12 in rat heart tissue with Caspase-12 antibody at 20 µg/ml.

Western blot analysis of Caspase-12 expression in rat heart tissue lysate with Caspase-12 antibody at (A) 0.25 and (B) 0.5 µg/ml.

Immunohistochemistry of Caspase-12 in mouse heart tissue with Caspase-12 antibody at 2 µg/ml.

Immunofluorescence of Caspase-12 in mouse heart tissue with Caspase-12 antibody at 20 µg/ml. Green: Caspase-12 Antibody (Large) (orb1239263) Blue: DAPI staining.