VSV-G Antibody

SKU: orb345400

Description

VSV-G antibody

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution Range: ELISA: 1:50,000 - 1:100,000, IHC: 1:200 - 1:500, IF: 1:200 - 1:500, WB: 1:1,000 - 1:5,000
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA and western blot. This product is suitable for immunofluorescence microscopy. Specific conditions for reactivity should be optimized by the end user. For standard indirect immunofluorescence assay we recommend paraformaldehyde fixation with detergent permeabilization of cells infected with VSV or transfected with the wild-type VSV-G protein. Staining is quite clean and specific for both sources of the G protein when diluted at least 1/200. At higher concentrations, a filamentous background staining may be present.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 501-511 of vesicular stomatitis virus glycoprotein (VSV-G).
• Purity: This affinity purified antibody is directed against VSV-G protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.19 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

Rabbit Anti-VSV-G Epitope Tag Antibody, Rabbit Anti VSV-G Epitope Tag Antibody, Rabbit Anti-VSV-G Tag Antibody

Western Blot of Rabbit anti-VSV-G antibody. Lane 1: 12 Epitope Tag Protein Marker Lysate - orb348609. Load: ~10 µg per lane. Primary antibody: VSV-G antibody at 1:1000 for overnight at 4°C. Secondary antibody: DyLight™ 649 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 3% BSA-TBS 2H at RT. Predicted/Observed size: ~50 kDa for VSV-G. Other band(s): VSV-G splice variants and isoforms.